试剂盒 > 酶联免疫试剂盒 > Human L-lactate dehydrogenase C chain(LDHC) ELISA kit
    • ELISA Kit
    • Human L-lactate dehydrogenase C chain(LDHC) ELISA kit
    • Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5 untranslated region.[provided by RefSeq]
    • UniProtP07864
    • BIOWWWLDHC
    • HGNC6544
    • RGD69366
    • MGI96764
    • OMIM150150
    • CUSABIOCSB-EL012844HU
  • 产品类型:
    ELISA Kit
  • 产品名称:

    Human L-lactate dehydrogenase C chain(LDHC) ELISA kit

  • 英文名称:

    Human L-lactate dehydrogenase C chain(LDHC) ELISA kit

  • 货号:
    CSB-EL012844HU
  • 别名:
    CT32, LDH3, LDHX, MGC111073, L-lactate dehydrogenase C|cancer/testis antigen 32
  • 规格:
    96T
  • 中文价格:
    3600
  • 种属:
    Homo sapiens (Human)
  • 其他种属:
  • 待测物名称:
    lactate dehydrogenase C
  • 缩写:
    LDHC
  • 蛋白功能1:
    Biosynthesis/Metabolism
  • 蛋白功能2:
    glyconeogenesis and glycometabolism
  • 蛋白功能3:
    Glycolysis
  • 检测范围:
    6.25 mU/mL-400 mU/mL
  • 灵敏度:
    1.56 mU/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected
    400 2.114 2.204 2.159 1.991
    200 1.636 1.604 1.620 1.452
    100 1.145 1.241 1.193 1.025
    50 0.787 0.778 0.783 0.615
    25 0.572 0.568 0.570 0.402
    12.5 0.339 0.361 0.350 0.182
    6.25 0.274 0.268 0.271 0.103
    0 0.169 0.167 0.168  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human LDHC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 86
    Range % 82-89
    1:2 Average % 91
    Range % 87-95
    1:4 Average % 102
    Range % 98-106
    1:8 Average % 96
    Range % 92-100
  • 回收率:
    The recovery of human LDHC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 85 81-89
    EDTA plasma (n=4) 93 90-96
  • 定性/定量:
    quantitative
  • 研究领域:
    Metabolism